If autoclaves is new to you, you might not know what to look for in a machine. This article will assist you in selecting the ideal autoclave for your requirements, taking into account factors like temperature, self-sealing, and vacuum pump. We’ll also discuss nitrogen generators’ advantages and disadvantages as well as other unique aspects. You should be aware that autoclaves can create either hot or cold liquids.


You’ll require a vacuum pump if you’re operating in an autoclave. Compressed air is released from these devices through a liquid seal inside the chamber. A seal constructed of water encloses the air as the pump rotates, producing a superior vacuum. It’s crucial to comprehend how an autoclave operates before using one because the temperature of the water seal varies depending on the type of autoclave. It will be easier for you to avoid issues and downtime if you have a basic understanding of how they operate.


The temperature of autoclaves is crucial in industrial applications to guarantee the security of materials and objects. These devices eliminate hazardous microorganisms by applying pressure to steam. The moisture in the steam, which is transferred with the heat and steam, breaks down the protein structure of bacteria. The optimal temperature for each of the several types of autoclaves changes according to the cycle. 121 degrees Celsius, or 250 degrees Fahrenheit, is considered the norm. Despite being significantly higher than the boiling point of water, this temperature is still below what is needed to completely sterilise an object.

Nitrogen Generators

Autoclave nitrogen generators come in two different varieties. The first pushes the gas through using specialised membrane materials that serve as filters. Over 90% of the air molecules are pushed out by the membrane, leaving just pure nitrogen. The membrane is protected and additional particles are removed in more sophisticated systems using multiple filtration stages. Air dryers and fine mesh filters are a couple of them. The generated nitrogen is then fully pressurised and supplied into an on-site receiver tank.

Horizontal Cylindrical Autoclave

Proper Size

The easiest way to choose the proper size autoclave for your lab when comparing models is to look at the chamber capacity. The capacity of an autoclave chamber is often expressed in litres. The real chamber capacity, however, may be less. Due to the layout of the chambers and the quantity of racks present, this is the case. For your lab, you can still buy an enormous autoclave, though.


Maintenance is essential for the successful operation of an autoclave. It should be carried out by a trained maintenance specialist. A weekly door switch inspection should be performed by a maintenance specialist. Accordingly, the device needs to be adjusted. Never use it if the door switch hasn’t been adjusted. Make a call for assistance if a problem arises. For these equipment to work properly, autoclave maintenance is essential. Here are some guidelines for maintaining an autoclave.